Endocrine Abstracts

In this study, pituitary of five carp fish were collected. The pituitary cells were dispersed enzymatically and cultivated as monolayers in MEM for 72 h. Then the culture media were collected and frozen in −20 °C.The carp ovaries were also collected and their follicles were separated mechanically and incubated in BSS Cortland medium in 24 well microplates for 48 h in room temperature. Then the incubated ovarian follicles were divided in two gr...